Full set of entries for the image competition

Entry 1: Alicia Murcia, Dept of Veterinary Medicine

Primary bone marrow macrophages infected with a GFP-labeledSalmonella Typhimurium strain (JH3016) for 10 minutes. Immunostaining of early (EEA1) and late (LAMP-1) phagosome markers.

Entry 2: Ed Hutchinson, Dept of Pathology

Strain of influenza A where the virions, rather than being spherical, are extruded from the infected cell as very long filaments. Bundles of filaments can be seen streaming from the surface of an infected MDCK cell. (The image is taken by widefield immunofluorescence.)

Entry 3: Ed Hutchinson, Dept of Pathology

A highly magnified view of a plaque - regions of a 'lawn' of cells in which a single virus has initiated multiple rounds of infection until the infected area is large enough to see by eye.

Here, cell nuclei are stained blue with DAPI, one of the viral proteins is labelled green and the RNA of of the segments of influenza A's genome is labelled red.

Entry 4: Ed Hutchinson, Dept of Pathology

A highly magnified view of a plaque - regions of a 'lawn' of cells in which a single virus has initiated multiple rounds of infection until the infected area is large enough to see by eye.

Here, cell nuclei are stained blue with DAPI, one of the viral proteins is labelled green and the RNA of the segments of influenza A's genome is labelled red.

Entry 5: Daniel Horton, Dept of Zoology

Antigenic cartography techniques help improve the resolution of cross-neutralisation data, reliably quantifying the antigenic variation between viruses and allowing visualisation of their antigenic relationships. This three dimensional image shows the antigenic relationships of a diverse panel of lyssaviruses. The 3d graphics were made using PyMOL Molecular Graphics System (DeLano WL 2002).

Entry 6: Daniel Horton, Dept of Zoology.

Antigenic cartography techniques help improve the resolution of cross-neutralisation data, reliably quantifying the antigenic variation between viruses and allowing visualisation of their antigenic relationships. This three dimensional image shows the antigenic relationships of a diverse panel of lyssaviruses. The 3d graphics were made using PyMOL Molecular Graphics System (DeLano WL 2002

Entry 7: James Hutchinson, Dept. of Pathology

Two human Mel-Juso cells infected with GFP-expressing Salmonella typhimurium (green), which are seen replicating in vacuoles within the cells which accumulate and induce tubular projections of CD1B-positive compartments (white). CD1B is involved in lipid antigen presentation to T cells. The late endosomal marker Lamp1 is shown in red and the nuclei in blue, while the cell outlines from phase contrast imaging are shown in grey on the grid below. Each colour channel was rendered in 3D from confocal z-stack data using direct volume rendering via the free Image Surfer software, before compiling and conversion to jpeg format in Photoshop.

Entry 8: Dr Mike Gill, Dept of Pathology

MHV68 TK truncation; Cells expressing a truncation mutant of

eGFP-MHV-68 Thymidine kinase.

Entry 9: Dr Mike Gill, Dept of Pathology

eGFPMHV68TK truncations: Cells expressing truncation mutants of

eGFP-MHV-68 Thymidine kinase. (need to open in photoshop to see well).

Entry 10: Dr Mike Gill, Dept of Pathology

gp48 virus cells: Cells infected with MHV-68 stained for the viral glycoprotein gp48. (Need to open in photoshop to see well).

Entry 11: Dr Mike Gill, Dept of Pathology

gORF58_gp48: Cells infected with a fluorescent recombinant MHV-68 virus expressing eGFP-ORF58 and co-stained for the viral glycoprotein (gp48).

Entry 12: Pablo Murcia, Dept of Veterinary Medicine

Cells expressing enJS56A1, an endogenous retrovirus of sheep. Green: virus tagged with an epitope; red: same virus tagged with another epitope. Image taken as a positive control for colocalization experiments, with yellow indicating that the two viruses go to the same place within the cell.

Entry 13: Agi Foglein, Dept of Pathology

Chicken fibroblast infected with a low pathogenicity avian influenza virus. Stained for DNA (blue), viral polymerase (green) and viral nucleoprotein (red).

Entry 14: Eliot Read, Dept of Pathology

MDCK cells infected with a mutant human strain of virus that probably has a defect in packaging its genome.  The cells are stained for DNA (blue) and two separate segments of the viral genome (by fluorescent in situ hybridisation) in red and green. Several cells only stain red indicating aborted infection because of lack of the genes encoded by the missing segment.

Entry 15: Eva Kreysa, Dept of Pathology

MDCK cells infected with a human virus, stained for DNA (blue), the viral nucleoprotein (green) and a nucleolar protein (nucleophosmin) in red. Note that in infected cells, the nucleolar staining pattern is disrupted. 

Entry 16: Dr Ian Brierley, Dept. of Pathology

A new model of how mRNA pseudoknot structures bring about ribosomal frameshifting during viral protein synthesis. A representation of the stalled complexes, with the ribosomal subunits in beige (40S) and blue (60S), the tRNA in green, eEF2 in red and the pseudoknot in purple.

Entry 17: Dr Ian Brierley & Oliver Long, Dept of Pathology

Stylised image of how mRNA pseudoknot structures bring about ribosomal frameshifting during viral protein synthesis. Ice block represents the cryo-electron microscopy used to image the ribosomes, the background a compilation of different viral pseudoknots.

Entry 18: Joao Proenca, Dept of Pathology

Sensory neurones (in blue) harbouring latent herpes simplex virus. Infected reporter mice encode reporter genes that are marked following infection with recombinant viruses expressing Cre recombinase. The betagalactosidase enzyme produces the blue staining which migrates out of the nerve cell body to label axons/nerve fibres.

Entry 19: Mark Sheppard, Dept of Veterinary Medicine

Multifluorescent shot of a Salmonella infected cell. Green channel isn't GFP as it was really poorly expressed. Red: anti-GFP ab (Alexa 568 in CY3.5 Filter) Yellow: anti-Cre ab (Alexa 647 in CY5 Filter) Phase shot with bacteria adhering to cell membrane.

Entry 20: Mark Sheppard, Dept of Veterinary Medicine

Artery. Green and red channels are autofluoresence from the connective and elastic tissue. Blue: nucleus (dapi).

Entry 21: Mark Sheppard, Dept of Veterinary Medicine

Salmonella about to be engulfed by macrophage. Salmonella -anti-04 Salmonella LPS antibody /Alexa Fluor 488. anti-CD18 antibody (Yellow -CY5 Filter). Blue: nuclei (dapi)

Entry 22: Mark Sheppard, Dept of Veterinary Medicine

Cells after prolonged contact with Salmonella LPS phase. Yellow: cell membrane. Blue: nucleus (dapi).

Entry 23: Mark Sheppard, Dept of Veterinary Medicine

Cells in the chicken bursa. Green: putative anti-CD83 antibody/ Alexa fluor 488. Red: anti-IgM /Alexa Fluor 568. Nuclei stained with dapi

Entry 24: Mark Sheppard, Dept of Veterinary Medicine

Cells in the chicken bursa. Green: putative CD83 cells (anti-CD83 / Alexa Flour 488). Red: MHCII cells (anti-MHCII / Alexa Fluor 568)

Entry 25: Mark Sheppard, Dept of Veterinry Medicine

Caspase 3 positive cell (in red, stained with anti-Caspase 3 antibody conjugated to Alexa Fluor 568). Bacterium stained with anti-salmonella 04 LPS antibody. Nuclei stained with dapi

Entry 26: Mark Sheppard, Dept of Veterinary Medicine

Neutrophils with Bordetella Toxin. Anti-9D4 antibody was conjugated to Alexa 488 to visualise the toxin. Nuclei stained with dapi

Entry 27: Mark Sheppard, Dept of Veterinary Medicine

Composite image

Entry 28: Mark Sheppard, Dept of Veterinary Medicine

Composite image

Entry 29: Mark Sheppard, Dept of Veterinary Medicine

Bacterial art on a Petri dish

Entry 30: Martin Welch, Dept of Biochemistry

Bacterial art on a Petri dish

Entry 31: Professor David Dunne, Dept of Pathology

AdultSchistosoma mansoni worms in the blood vessels around the intestine

 

Posted on Thursday 20 December, 2007