We are interested in the molecular and structural biology of how RNA viruses package their genetic material and how they select their own RNA from the cell so specifically.
We study two major groups of viruses ; lentiviruses (retroviruses) and rotaviruses. The lentiviruses include HIV-1, HIV-2 and FIV. In all of these viruses we are interested in virus assembly, how the virus captures its RNA genome within the cell and how it uses cellular machinery to do this. The work involves structural biology, confocal microscopy and FRET as well as molecular biological techniques. We collaborate with structural biologists in Cambridge and Oxford. We also use our understanding of lentiviral RNA packaging to generate gene vectors for gene transfer and gene therapy purposes.
Departments and Institutes
Anderson EC, Lever AML (2006), HIV-1 Gag polyprotein modulates its own translation, Journal of Virology 80: 10478–10486
Poole E, Strappe P, Mok H-P, Hicks R, Lever AML, (2005), HIV-1 Gag-RNA interaction occurs at a perinuclear/centrosomal site; analysis by confocal microscopy and FRET, Traffic 6: 741–55
Lever AML (2007), “HIV-1 RNA Packaging”, Advances in Pharmacology, 55:1–32