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Cambridge Infectious Diseases

An Interdisciplinary Research Centre at the University of Cambridge
 

Biography

I am currently a Herchel Smith postdoctoral research fellow at the Department of Biochemistry, University of Cambridge. My research aims to uncover the molecular mechanisms that govern interkingdom interactions during polymicrobial infection.  

Following my undergraduate degree in Biological and Biomedical Sciences at Maynooth University (2008-2012), I carried out my PhD in Biotechnology at Maynooth under the direction of Prof. Sean Doyle (2012-2016). I also visited the laboratories of Prof. Gustavo Goldman at the University of São Paulo (2014) and Prof. Wulf Blankenfeldt at the Helmholtz Centre for Infection Research, Germany (2015). For my PhD I characterised novel regulators of toxin production in the pathogenic fungus Aspergillus fumigatus and explored their potential applications in diagnostics and biotechnology. 

Research

I have a keen interest in both the mechanistic and regulatory aspects of virulence in opportunistic human pathogens.

Publications

Key publications: 

 

Dolan S.K., (2020) Current Knowledge and Future Directions in Developing Strategies to Combat Pseudomonas aeruginosa Infection. Journal of Molecular Biology. doi: 10.1016/j.jmb.2020.07.021

Dolan S.K., Kohlstedt, M., Trigg, M., Vallejo Ramirez, P., Kaminski, P.F., Wittmann, C., Welch, M., (2020) Contextual flexibility in Pseudomonas aeruginosa central carbon metabolism during growth in single carbon sources. mBio. doi: 10.1128/mBio.02684-19

Dolan S.K, Pereira. G.P., Silva-Rocha, R. and Welch, M. (2019) Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa. Microbial Biotechnology. doi: 10.1111/1751-7915.13423

Doyle, S., Jones, G.W, and Dolan S.K. (2017) Dysregulated gliotoxin biosynthesis attenuates the production of unrelated metabolites in Aspergillus fumigatus. Fungal Biology. doi: 10.1016/j.funbio.2017.12.007. 

Dolan S.K, Bock, T., Hering, V., Jones, G.W, Blankenfeldt W., Doyle S. (2017) Structural, mechanistic and functional insight into gliotoxin bis-thiomethylation in Aspergillus fumigatus. Open Biology. doi: 10.1098/rsob.160292

Dolan, S. K., Owens, R. A., O’Keeffe, G., Hammel, S., Fitzpatrick, D. A, Jones, G. W., and Doyle, S. (2014) Regulation of Nonribosomal Peptide Synthesis: bis-Thiomethylation Attenuates Gliotoxin Biosynthesis in Aspergillus fumigatus. Cell Chemistry and Biology. 21, 999–1012. doi:10.1016/j.chembiol.2014.07.006.

 

Other publications: 

McNamara, L., Dolan, S.K., Walsh, J.M., Stephens, J.C., Glare, T.R., Kavanagh, K., Griffin, C.T. (2019) Oosporein, an abundant metabolite in Beauveria caledonica, with a feedback induction mechanism and a role in insect virulence. Fungal Biology. doi: 10.1016/j.funbio.2019.01.004

Crousilles, A., Dolan S.K., Brear, P., Chirgadze, D.Y., and Welch, M. (2018) Gluconeogenic precursor availability regulates flux through the glyoxylate shunt in Pseudomonas aeruginosa. J Biol Chem. doi: 10.1074/jbc.RA118.004514. 

Manfiolli, A. O., de Castro, P. A., dos Reis, T. F., Dolan, S.K., Doyle, S., Jones, G., … Goldman, G. H. (2017) Aspergillus fumigatus protein phosphatase PpzA is involved in iron assimilation, secondary metabolite production, and virulence. Cellular Microbiology. doi: e12770.

Mulvihill, E., Moloney, N., Owens, R.A., Dolan, S.K., Russell, L and Doyle, S (2017) Functional Characterization, Vacuolar Localization and Role of MirC in Iron Homeostasis in Aspergillus fumigatus. Front. Microbiol. doi: 10.3389/fmicb.2017.00418

Castro, P.A., Dos Reis, T.F, Dolan, S.K., Manfiolli, A.O., Brown, N.A., Jones, G.W., Doyle, S., Riaño-Pachón, D.M., Squina, F.M., Caldana, C., Singh, A., Poeta, M., Hagiwara, D., and Goldman, G.H. (2016) The Aspergillus fumigatus SchASCH9 kinase modulates SakAHOG1 MAP kinase activity and it is essential for virulence. Molecular Microbiology. doi: 10.1111/mmi.13484

Smith, E.B., Dolan, S.K., Fitzpatrick, D.A., Doyle, S., and Jones, G.W. (2016) Towards understanding the gliotoxin detoxification mechanism: in vivo thiomethylation protects yeast from gliotoxin cytotoxicity. Microbial Cell. doi: 10.15698/mic2016.03.485

Owens, R. A., O’Keeffe, G., Smith, E., Dolan, S. K., Hammel, S., Sheridan, K., Fitzpatrick, D. A, Jones, G. W., and Doyle, S. (2015) Interplay between Gliotoxin Resistance, Secretion and the Methyl/Methionine Cycle in Aspergillus fumigatus. Eukaryotic Cell. EC.00055-15.

Winkelströter, L.K., Dolan, S. K., Reis, T. F., Bom, V. L., Castro, P. A., Hagiwara, D., Alowni, R., Jones, G.W., Doyle S., Brown, N.A. and Goldman, G.H. (2015) Systematic Global Analysis of Genes Encoding Protein Phosphatases in Aspergillus fumigatus. G3: Genes, Genomes, Genetics. doi:10.1534/g3.115.016766

Dolan, S. K., O’Keeffe, G., Jones, G. W., and Doyle, S. (2015). Resistance is not futile: gliotoxin biosynthesis, functionality and utility. Trends in Microbiology. 1–10. doi:10.1016/j.tim.2015.02.005.

 

Herchel Smith Postdoctoral Fellow, Department of Biochemistry
Dr Stephen  Kevin Dolan

Contact Details

University of Cambridge, Department of Biochemistry
Hopkins Building, Downing Site
Tennis Court Road
Cambridge
CB2 1QW
Available for consultancy

Affiliations

Classifications: 
Departments and institutes: 
Person keywords: 
Genomics
Molecular Biology
Host-Pathogen Interaction
Infection
Transcriptomics
Enzyme
Virulence
Pathogenesis
Sequencing
Drug Discovery
Metabolism
Gene Regulation